125. The fluid membrane determines mechanics of erythrocyte extracellular vesicles and is softened in hereditary spherocytosis
Vorselen, D; van Dommelen, SM; Sorkin, R; Piontek, MC; Schiller, J; Dopp, ST; Kooijmans, SAA; van Oirschot, BA; Versluijs, BA; Bierings, MB; van Wijk, R; Schiffelers, RM; Wuite, GJL; Roos, WH
NATURE COMMUNICATIONS Volume: 9 Article Number: 4960 (2018)

124. Effect of dsDNA on the Assembly Pathway and Mechanical Strength of SV40 VP1 Virus-like Particles
van Rosmalen, MGM; Li, CL; Zlotnick, A; Wuite, GJL; Roos, WH  
BIOPHYSICAL JOURNAL Volume: 115  Issue: 9  Pages: 1656-1665 (2018)

123. Nanomechanics of Extracellular Vesicles Reveals Vesiculation Pathways
Sorkin, R; Huisjes, R; Boskovic, F; Vorselen, D; Pignatelli, S; Ofir-Birin, Y; Leal, JKF; Schiller, J; Mullick, D; Roos, WH; Bosman, G; Regev-Rudzki, N; Schiffelers, RM; Wuite, GJL  
SMALL Volume: 14  Issue: 39 Article Number: 1801650 (2018)

122. Single-Cell Acoustic Force Spectroscopy: Resolving Kinetics and Strength of T Cell Adhesion to Fibronectin
Kamsma, D; Bochet, P; Oswald, F; Alblas, N; Goyard, S; Wuite, GJL; Peterman, EJG; Rose, T 
CELL REPORTS Volume: 24  Issue: 11  Pages: 3008-3016 (2018)

121. Reconstitution of anaphase DNA bridge recognition and disjunction
Sarlos, K; Biebricher, AS; Bizard, AH; Bakx, JAM; Ferrete-Bonastre, AG; Modesti, M; Paramasivam, M; Yao, Q; Peterman, EJG; Wuite, GJL; Hickson, ID
NATURE STRUCTURAL & MOLECULAR BIOLOGY Volume: 25  Issue: 9  Pages: 868 (2018)

120. Probing cellular mechanics with acoustic force spectroscopy
Sorkin, R; Bergamaschi, G; Kamsma, D; Brand, G; Dekel, E; Ofir-Birin, Y; Rudik, A ; Gironella, M; Ritort, F; Regev-Rudzki, N; Roos, WH; Wuite, GJL  
MOLECULAR BIOLOGY OF THE CELL Volume: 29  Issue: 16  Pages: 2005-2011  (2018)

119.  Viscoelastic properties of vimentin originate from nonequilibrium conformational changes
Block, J; Witt, H; Candelli, A; Danes, JC; Peterman, EJG; Wuite, GJL; Janshoff, A; Koster, S  
SCIENCE ADVANCES Volume: 4  Issue: 6 Article Number: eaat1161 (2018)

118.  A single point mutation in precursor protein VI doubles the mechanical strength of human adenovirus
van Rosmalen, MGM; Nemerow, GR; Wuite, GJL; Roos, WH  
JOURNAL OF BIOLOGICAL PHYSICS Volume: 44  Issue: 2  Pages: 119-132  (2018)

117. Acetylation and phosphorylation of human TFAM regulate TFAM-DNA interactions via contrasting mechanisms
King, GA; Shabestari, MH; Taris, KKH; Pandey, AK; Venkatesh, S; Thilagavathi, J; Singh, K; Koppisetti, RK; Temiakov, D; Roos, WH; Suzuki, CK; Wuite, GJL  
NUCLEIC ACIDS RESEARCH Volume: 46  Issue: 7  Pages: 3633-3642 (2018)

116. Two distinct conformational states define the interaction of human RAD51-ATP with single-stranded DNA
Brouwer, I; Moschetti, T; Candelli, A; Garcin, EB; Modesti, M; Pellegrini, L; Wuite, GJL; Peterman, EJG  
EMBO JOURNAL Volume: 37  Issue: 7 Article Number: UNSP e98162 (2018)

115. Quantifying Local Molecular Tension Using Intercalated DNA Fluorescence
King, GA; Biebricher, AS; Heller, I; Peterman, EJG; Wuite, GJL  
NANO LETTERS Volume: 18  Issue: 4  Pages: 2274-2281 (2018)

 114. A polarized view on DNA under tension
van Mameren, J; Vermeulen, K; Wuite, GJL; Peterman, EJG  
JOURNAL OF CHEMICAL PHYSICS Volume: 148  Issue: 12 Article Number: 123306 (2018)

113. Multilamellar nanovesicles show distinct mechanical properties depending on their degree of lamellarity
Vorselen, D; Marchetti, M; Lopez-Iglesias, C; Peters, PJ; Roos, WH; Wuite, GJL  
NANOSCALE Volume: 10  Issue: 11  Pages: 5318-5324 (2018)

 112. Programming the mechanics of cohesive fiber networks by compression
Vos, BE; Liebrand, LC; Vahabi, M; Biebricher, A; Wuite, GJL; Peterman, EJG; Kurniawan, NA; MacKintosh, FC; Koenderink, GH  
SOFT MATTER Volume: 13  Issue: 47  Pages: 8886-8893 (2017)

111. Hyperstretching DNA
Schakenraad, K; Biebricher, AS; Sebregts, M; ten Bensel, B; Peterman, EJG; Wuite, GJL; Heller, I; Storm, C; van der Schoot, P  
NATURE COMMUNICATIONS Volume: 8 Article Number: 2197 (2017)

110. Probing the mechanical stability of bridged DNA-H-NS protein complexes by single-molecule AFM pulling
Liang, Y; van der Valk, RA; Dame, RT; Roos, WH; Wuite, GJL  
SCIENTIFIC REPORTS Volume: 7 Article Number: 15275 (2017)

109. Supported Planar Mammalian Membranes as Models of in Vivo Cell Surface Architectures
Liu, HY; Grant, H; Hsu, HL; Sorkin, R; Boskovic, F; Wuite, G; Daniel, S  
ACS APPLIED MATERIALS & INTERFACES Volume: 9  Issue: 41  Pages: 35526-35538 (2017)

108. How to switch the motor on: RNA polymerase initiation steps at the single-molecule level
Marchetti, M; Malinowska, A; Heller, I; Wuite, GJL  
PROTEIN SCIENCE Volume: 26  Issue: 7  Pages: 1303-1313  (2017)

107. Vertex-Specific Proteins pUL17 and pUL25 Mechanically Reinforce Herpes Simplex Virus Capsids
Snijder, J; Radtke, K; Anderson, F; Scholtes, L; Corradini, E; Baines, J; Heck, AJR; Wuite, GJL; Sodeik, B; Roos, WH
JOURNAL OF VIROLOGY Volume: 91  Issue: 12 (2017)

106. Human RAD52 Captures and Holds DNA Strands, Increases DNA Flexibility, and Prevents Melting of Duplex DNA: Implications for DNA Recombination
Brouwer, I; Zhang, H; Candelli, A; Normanno, D; Peterman, EJG; Wuite, GJL; Modesti, M  
CELL REPORTS Volume: 18  Issue: 12  Pages: 2845-2853 (2017)

105. Single-molecule observation of DNA compaction by meiotic protein SYCP3
Syrjanen, JL; Heller, I; Candelli, A; Davies, OR; Peterman, EJG; Wuite, GJL; Pellegrini, L
ELIFE Volume: 6 Article Number: e22582 (2017)

104. Competition between Bending and Internal Pressure Governs the Mechanics of Fluid Nanovesicles
Vorselen, D; MacKintosh, FC; Roos, WH; Wuite, GJL  
ACS NANO Volume: 11  Issue: 3  Pages: 2628-2636 (2017)

 103. Switching between Exonucleolysis and Replication by T7 DNA Polymerase Ensures High Fidelity
Hoekstra, TP; Depken, M; Lin, SN; Cabanas-Danes, J; Gross, P; Dame, RT; Peterman, EJG; Wuite, GJL
BIOPHYSICAL JOURNAL Volume: 112  Issue: 4  Pages: 575-583 (2017)

102. Nonlinear Loading-Rate-Dependent Force Response of Individual Vimentin Intermediate Filaments to Applied Strain
Block, J; Witt, H; Candelli, A; Peterman, EJG; Wuite, GJL; Janshoff, A; Koster, S.
PHYSICAL REVIEW LETTERS Volume: 118  Issue: 4 Article Number: 048101 (2017)

101. Recent Advances in Biological Single-Molecule Applications of Optical Tweezers and Fluorescence Microscopy
Shabestari, MH; Meijering, AEC; Roos, WH; Wuite, GJL; Peterman, EJG
Methods in Enzymology Volume: 582  Pages: 85-119 (2017)

100. Controlled tip wear on high roughness surfaces yields gradual broadening and rounding of cantilever tips
Vorselen, D; Kooreman, ES; Wuite, GJL; Roos, WH
SCIENTIFIC REPORTS Volume: 6 Article Number: 36972 (2016)

99. Atomic force microscopy observation and characterization of single virions and virus-like particles by nano-indentation
Marchetti, M.; Wuite, G. J. L.; Roos, W. H.
CURRENT OPINION IN VIROLOGY   Volume: 18   Pages: 82-88 (2016)

98. Fibrin Networks Support Recurring Mechanical Loads by Adapting their Structure across Multiple Scales
Kurniawan, Nicholas A.; Vos, Bart E.; Biebricher, Andreas; ; Wuite, Gijs J. L.; Peterman, Erwin J. G.; Koenderink, Gijsje H.
BIOPHYSICAL JOURNAL   Volume: 111   Issue: 5   Pages: 1026-1034 (2016)

97. Sliding sleeves of XRCC4-XLF bridge DNA and connect fragments of broken DNA
Ineke Brouwer, Gerrit Sitters, Andrea Candelli, Stephanie J. Heerema, Iddo Heller, Abinadabe J. de Melo, Hongshan Zhang, Davide Normanno, Mauro Modesti, Erwin J.G. Peterman, Gijs J.L. Wuite
NATURE  Volume: 535   Issue: 7613   Pages: 566 (2016)

96. Mechanical Unfolding of an Autotransporter Passenger Protein Reveals the Secretion Starting Point and Processive Transport Intermediates
Baclayon, Marian; van Ulsen, Peter; Mouhib, Halima;  Shabestari, Maryam Hashemi; Verzijden, Timo; Abeln, Sanne; Roos, Wouter H.; Wuite, Gijs J. L.
ACS NANO   Volume: 10   Issue: 6   Pages: 5710-5719 (2016)

95. Protein Nanocontainers from Nonviral Origin: Testing the Mechanics of Artificial and Natural Protein Cages by AFM
Heinze, K.; Sasaki, E.; King, N. P.; Baker, D.; Hilvert, D.; Wuite, G. J. L.; Roos, W. H.
JOURNAL OF PHYSICAL CHEMISTRY B   Volume: 120   Issue: 26   Pages: 5945-5952 (2016)

94. Tuning the Music: Acoustic Force Spectroscopy (AFS) 2.0
Kamsma, Douwe; Creyghton, Ramon; Sitters, Gerrit;  Wuite, Gijs J. L.Peterman, Erwin J G
METHODS   Volume: 105   Pages: 26-33  (2016)

93. DNA twist stability changes with magnesium(2+) concentration
Onno D. Broekmans, Graeme A. King, Greg J. Stephens, and Gijs J.L. Wuite
PHYS. REV. LETT.  Volume: 116   Issue: 25  (2016)

92. Assembly and Mechanical Properties of the Cargo-Free and Cargo Loaded Bacterial Nanocompartment Encapsulin
Snijder, Joost; Kononova, Olga; Barbu, Ioana M.;  Uetrecht, Charlotte; Rurup, W. Frederik; Burnley, Rebecca J.; Koay, Melissa S. T.; Cornelissen, Jeroen J. L. M.; Roos, Wouter H.; Barsegov, Valeri; Wuite, Gijs J. L.; Heck, Albert J. R.
BIOMACROMOLECULES   Volume: 17   Issue: 8   Pages: 2522-2529 (2016)

91. Diverse architectural properties of Sso10a proteins: Evidence for a role in chromatin compaction and organization
riessen, Rosalie P. C.; Lin, Szu-Ning; Waterreus, Willem-Jan;  van der Meulen, Alson L. H.; van der Valk, Ramon A.; Laurens, Niels; Moolenaar, Geri F.; Pannu, Navraj S.; Wuite, Gijs J. L.; Goosen, Nora; Dame, Remus T.
SCIENTIFIC REPORTS   Volume: 6 (2016)

90. Unravelling the structural plasticity of stretched DNA under torsional constraint.
King, Graeme A; Peterman, Erwin J G; Wuite, Gijs J L
NATURE COMMUNICATIONS Volume: 7 Pages: 11810 (2016)

89. Optical Pushing: A Tool for Parallelized Biomolecule Manipulation.
Sitters, G; Laurens, N; de Rijk, EJ; Kress, H; Peterman, EJG; Wuite, GJL
BIOPHYSICAL JOURNAL   Volume: 110   Issue: 1   Pages: 44-50  (2016)

88. Fluctuating Nonlinear Spring Model of Mechanical Deformation of Biological Particles. 
Kononova, O; Snijder, J; Kholodov, Y; Marx, KA; Wuite, GJL; Roos, WH; Barsegov, V
PLOS COMPUTATIONAL BIOLOGY Volume: 12  Issue: 1  DOI: 10.1371/journal.pcbi.1004729 (2016)

87. Direct quantitative detection of Doc2b-induced hemifusion in optically trapped membranes.
Brouwer, I; Giniatullina, A; Laurens, N; van Weering, JRT; Bald, D; Wuite, GJL; Groffen, AJ
NATURE COMMUNICATIONS Volume: 6 Article Number: 8387 DOI: 10.1038/ncomms9387 (2015)

86. Biebricher, Andreas S.; Heller, Iddo; Roijmans, Roel F. H.; Hoekstra, Tjalle P.; Peterman, Erwin J. G.; Wuite, Gijs J. L. 
The impact of DNA intercalators on DNA and DNA-processing enzymes elucidated through force-dependent binding kinetics
NATURE COMMUNICATIONS 6:7304 DOI:10.1038/ncomms8304 (2015)

85. Sitters, Gerrit; Kamsma, Douwe; Thalhammer, Gregor; Ritsch-Marte, Monika; Peterman, Erwin J. G.; Wuite, Gijs J. L.
Acoustic force spectroscopy
NATURE METHODS 12:1 (2015)

84. Driessen, Rosalie P. C.; Sitters, Gerrit; Laurens, Niels; Moolenaar, Geri F.; Wuite, Gijs J. L.; Goosen, Nora; Dame, Remus Th.
Effect of Temperature on the Intrinsic Flexibility of DNA and Its Interaction with Architectural Proteins
BIOCHEMISTRY 53:41 (2014)

83. Candelli, Andrea; Holthausen, Jan Thomas; Depken, Martin; Brouwer, Ineke; Franker, Mariella A. M.; Marchetti, Margherita; Heller, Iddo; Bernard, Stephanie; Garcin, Edwige B.; Modesti, Mauro; Wyman, Claire; Wuite, Gijs J. L.; Peterman, Erwin J. G.
Visualization and quantification of nascent RAD51 filament formation at single-monomer resolution
Proc. Natl. Acad. Sci. U. S. A. 111:42 (2014)

82. Farge, Geraldine; Mehmedovic, Majda; Baclayon, Marian; van den Wildenberg, Siet M. J. L.; Roos, Wouter H.; Gustafsson, Claes M.; Wuite, Gijs J. L.; Falkenberg, Maria.
In Vitro-Reconstituted Nucleoids Can Block Mitochondrial DNA Replication and Transcription
CELL REPORTS 8:1 (2014)

81. Muiznieks, Lisa D.; Cirulis, Judith T.; van der Horst, Astrid; Reinhardt, Dieter P.; Wuite, Gijs J. L.; Pomes, Regis; Keeley, Fred W.
Modulated growth, stability and interactions of liquid-like coacervate assemblies of elastin

80. Heller, Iddo; Sitters, Gerrit; Broekmans, Onno D.; Biebricher, Andreas S.; Wuite, Gijs J. L.; Peterman, Erwin J. G. 
Mobility Analysis of Super-Resolved Proteins on Optically Stretched DNA: Comparing Imaging Techniques and Parameters
CHEMPHYSCHEM 15:4 (2014)

79. I. Heller, T.P. Hoekstra, G.A. King , E.J.G. Peterman, G.J.L. Wuite.
Optical Tweezers Analysis of DNA–Protein Complexes
Chem. Rev., 114:6 DOI:10.1021/cr4003006 (2014)

78. D. Luque, A. de la Escosura, J. Snijder, M. Brasch, R.J. Burnley, M.S.T. Koay, J.L. Carrascosa, G.J.L. Wuite, W.H. Roos, A.J.R. Heck, J. L. M. Cornelissen, T. Torres, J.R. Caston
Self-assembly and characterization of small and monodisperse dye nanospheres in a protein cage
Chemical Science 5(2):575-581 (2014)

77. G. King and G.J.L. Wuite
Nature Chemistry 6(1):13-14 (2014) – News and Views

76. Andrea Candelli, Mauro Modesti, Erwin J. G. Peterman, Gijs J. L. Wuite
Single-molecule views on homologous recombination
Quarterly Reviews of Biophysics 46:4 (2013)

75. A. Candelli, T.P. Hoekstra, G. Farge, P. Gross, E.J.G. Peterman, G.J.L. Wuite.
A toolbox for generating single-stranded DNA in optical tweezers experiments
Biopolymers 99(9) 611-620 (2013)

74. D. Vorselen, W.H. Roos, F.C. MacKintosh, G.J.L. Wuite, J.J.W.A. van Loon.
The role of the cytoskeleton in sensing changes in gravity by non-specialized cells.
FASEB J. doi: 10.1096/fj.13-236356 (2013)

73. O. Kononova, J. Snijder, M. Brasch, J. Cornelissen, R.I. Dima, K.A. Marx, G.J.L. Wuite, W.H. Roos, V. Barsegov   
Structural Transitions and Energy Landscape for Cowpea Chlorotic Mottle Virus Capsid mechanics from nanomanipulation in vitro and in silico
Biophysical Journal 105:1893-1903 (2013)

72. Andreas Biebricher, Seiki Hirano, Jacqueline H. Enzlin, Nicola Wiechens, Werner W. Streicher, Diana Huttner, Lily H.-C. Wang, Erich A. Nigg, Tom Owen-Hughes, Ying Liu, Erwin Peterman*, Gijs J.L. Wuite* and Ian D. Hickson*     
PICH: A DNA Translocase Specially Adapted for Processing Anaphase Bridge DNA
Molecular Cell, 10.1016/j.molcel.2013.07.016 (2013)

71. Iddo Heller, Gerrit Sitters, Onno D Broekmans, Géraldine Farge, Carolin Menges, Wolfgang Wende,     Stefan W Hell, Erwin J G Peterman & Gijs J L Wuite     
STED nanoscopy combined with optical tweezers reveals protein dynamics on densely covered DNA
Nature Methods, 10.1038/nmeth.2599 (2013) + Cover

See also: 
NRC (premier Dutch newspaper), 17 Aug. 2013, 11 Aug. 2013

70. J. Snijder, C. Uetrecht, R.J. Rose, R. Sanchez-Eugenia, G.A. Marti, J. Agirre, D.M.A. Guérin, G.J.L. Wuite, A.J.R. Heck, W.H. Roos     
Probing the biophysical interplay between a viral genome and its capsid
Nature Chemistry 5, 502-509 (2013)

 See also: Nature Chemistry News and ViewsBiophysical chemistry: Unravelling capsid transformations

69. Andrea Candelli, Tjalle Hoekstra, Geraldine Farge, Peter Gross, Erwin J.G. Peterman, Gijs J.L. Wuite    
A toolbox for generating single-stranded DNA in optical tweezers experiments
Biopolymers 99(9): 611-620 (2013)

68. G.A. King, P. Gross, U. Bockelmann, M. Modesti, G.J.L. Wuite*, E.J.G. Peterman*   
Revealing the Competition between Peeled-ssDNA, Melting Bubbles and S-DNA during DNA Overstretching using Fluorescence Microscopy
Proc. Natl. Acad. Sci. U. S. A. 110 (10) 3859-3864 (2013)

67. J. Snijder, V.S. Reddy, E.R. May, W.H. Roos, G.R. Nemerow, G.J.L. Wuite  
Integrin and defensin modulate the mechanical properties of adenovirus
Journal of Virology 87, 2756-2766 (2013) + Cover

66. N. Laurens, R.P.C. Driessen, I. Heller, D. Vorselen, M.C. Noom, F.J.H. Hol, M.F. White, R.T. Dame, G.J.L. Wuite     
Alba shapes the archaeal genome using a delicate balance of bridging and stiffening the DNA
Nature Communications  3:1328 (2012)

65. W. S. Klug, W. H. Roos, G.J.L. Wuite    
Unlocking Internal Prestress from Protein Nanoshells
Phys. Rev. Lett. 109:168104 (2012)     

See also: 
NRC (premier Dutch newspaper), 27 Oct. 2012
Physics (Selected highlights of the Physical Review journals)

64. G. Farge, N. Laurens, O.D. Broekmans, S.M.J.L. van den Wildenberg, L.C.M. Dekker, M. Gaspari, C.M. Gustafsson, E.J.G. Peterman, M. Falkenberg, G.J.L. Wuite     
Protein sliding and DNA denaturation are essential for DNA organization by human mitochondrial transcription factor A
Nature Communications 3:1013 (2012)   

63. W.H. Roos, I. Gertsman, E.R. May, C.L. Brooks, J.E. Johnson, G.J.L. Wuite    
Mechanics of bacteriophage maturation
Proc. Natl. Acad. Sci. U. S. A. 109(7):2342-2347 (2012)   

62. J. Snijder, I.L. Ivanovska, M. Baclayon, W.H. Roos, G.J.L. Wuite    
Probing the impact of loading rate on the mechanical properties of viral nanoparticles
Micron 43(12):1343-1350 (2012)   

61. D.A. Rusling, N. Laurens, C. Pernstich, I. Brouwer, G.J.L. Wuite, S.E. Halford    
DNA looping by FokI: the impact of synapse geometry on loop topology at varied site orientations
Nucleic Acid Research 40(11):4977-4987 (2012)  

60. N. Laurens, D.A. Rusling, C. Pernstich, I. Brouwer, S.E. Halford, G.J.L. Wuite     
DNA looping by FokI: the impact of twisting and bending rigidity on protein-induced looping dynamics
Nucleic Acid Research 40(11):4988-4997 (2012)  

59. D.A. Heuveling, G.W.M Visser,M. de Groot, J.F. de Boer, M. Baclayon, W.H. Roos, G.J.L. Wuite, C.R. Leemans, R. de Bree, G.A.M.S. van Dongen      
Nanocolloidal albumin-IRDye 800CW: a near-infrared fluorescent tracer with optimal retention in the sentinel lymph node
European Journal of Nuclear Medicine and Molecular Imaging 39(7):1161-1168 (2012)  

58. P. Gross, N. Laurens, L.B. Oddershede, U. Bockelmann, E.J.G. Peterman, G.J.L. Wuite     
Quantifying how DNA stretches, melts and changes twist under tension
Nature Physics 7(9):731-736 (2011)   

57. M. Baclayon, G.K. Shoemaker, C. Uetrecht, S.E. Crawford, M.K. Estes, B.V.V. Prasad, A.J.R. Heck, G.J.L. Wuite, W.H. Roos
Prestress Strengthens the Shell of Norwalk Virus Nanoparticles
Nano Letters 11(11):4865-4869 (2011)   

56. K. Sznee, J.P. Dekker, R.T. Dame, H. van Roon, G.J.L. Wuite, R.N. Frese     
Jumping Mode Atomic Force Microscopy on Grana Membranes from Spinach
Journal of Biological Chemistry 286(45):39164-39171 (2011)    

55. D.A. Heuveling, G.W.M. Visser, M. Baclayon, W.H. Roos, G.J.L. Wuite, O.S. Hoekstra, C.R. Leemans,R. de Bree, G.A.M.S. van Dongen    
Zr-89-Nanocolloidal Albumin-Based PET/CT Lymphoscintigraphy for Sentinel Node Detection in Head and Neck Cancer: Preclinical Results
Journal of Nuclear Medicine 52(10):1580-1584 (2011)   

54. I.L. Ivanovska, R. Miranda, J.L. Carrascosa, C.F. Schmidt*, G.J.L. Wuite*     
Discrete fracture patterns of virus shells reveal mechanical building blocks
Proc. Natl. Acad. Sci. U. S. A. 108(31):12611-12616 (2011)

53. A. Evilevitch, I.L. Ivanovska, W.H. Roos, M. Jeembaeva, B. Jönsson, G.J.L. Wuite      
Effects of salts on internal DNA pressure and mechanical stability of phages
Journal of Molecular Biology 405(1): 18-23 (2011) 

52. A. Candelli, G.J.L. Wuite, E.J.G. Peterman     
Combining optical trapping, fluorescence microscopy and micro-fluidics for single molecule studies of DNA-protein interactions
Physical Chemistry Chemical Physics 13(16):7263-7272 (2011)

51. W. H. Roos, R. Bruinsma G. J. L. Wuite     
Physical Virology
Nature Physics 6(10):733-743 (2010)

50. M. Baclayon, W.H. Roos, G.J.L. Wuite     
Sampling protein form and function using Atomic Force Microscope
Mol Cell Proteomics 9(8):1678-88 (2010)

49. W.H. Roos, M.M. Gibbons, A. Arkhipov, C. Uetrecht, N.R. Watts, P.T. Wingfield, A.C. Steven, A.J.R. Heck, K. Schulten, W.S. Klug, G.J.L. Wuite     
Deforming nanostructures: how continuum elastic models, molecular dynamics simulations and experiments coalesce at the nanoscale
Biophysical Journal 98(9):1902-1910 (2010)

Featuring as Research Highlight

48. M. Zaremba, A. Owsicka, G. Tamulaitis, G. Sasnauskas, L. Shlyakhtenko, A. Lushnikov, Y. Lyubchenko, N. Laurens, B. v/d Broek, G.J.L. Wuite, V. Siksnys
DNA synapsis through the transient tetramerization triggers the cleavage by Ecl18kI restriction enzyme
Nucleic Acids Research 38(20):7142-7154 (2010)

Article features as outstanding paper

47. J. van Mameren, G.J.L. Wuite, I. Heller
Introduction to optical tweezers: background, system designs, and commercial solutions
Chapter to be published in Methods in Molecular Biology: Single-Molecule techniques, Wuite, G.J.L. & Peterman, E.J.G. (Eds.), Humana Press

46. M. Baclayon, G. J. L. Wuite, W. H. Roos
Imaging and manipulation of single viruses by atomic force microscopy
Soft Matter 21:5273-5285 (2010) cover

45. G. K. Shoemaker, E. van Duijn, S.E. Crawford, C. Uetrecht, M. Salumbides-Baclayon, W.H. Roos, G.J.L. Wuite, M.K. Estes, B.V. Venkataram Prasad, A.J.R. Heck
Norwalk virus assembly and stability monitored by mass spectrometry
Mol Cell Proteomics 9(8):1742-51 (2010)

44. R.J. Kroeze, M.N. Helder, W.H. Roos, G.J.L. Wuite, V. Everts,  R.A. Bank, T.H. Smit
The effect of ethylene oxide, glow discharge and electron beam on the surface characteristics of poly(l-lactide-co-caprolactone) and the corresponding cellular response of adipose stem cells
Acta Biomaterialia 6(6):2060-2065 (2010) doi:10.1016/j.actbio.2009.11.022

43. W.H. Roos and G.J.L. Wuite
Mechanical properties of viruses
In Emerging Topics in Physical Virology, Stockley, P. & Twarock, R. (Eds.), London: Imperial College Press (2010) 

42. B. van den Broek, M.C. Noom, J. van Mameren, C. Battle, F.C. MacKintosh, G.J.L. Wuite
Visualizing the formation and collapse of DNA toroids
Biophysical Journal 98(9):1902-10 (2010)

41. R Metzler, B van den Broek, G.J.L. Wuite, M.A. Lomholt
Role of DNA conformation in gene regulation
In Biophysics of DNA-protein interactions: From single molecules to biological systems, Williams, M & Maher, J (Eds.), Springer (2010)

40. P. Gross, G. Farge, E.J.G. Peterman, G.J.L. Wuite
Combining optical tweezers, single molecule fluorescence microscopy and microfluidics for studies of DNA-proteins interactions
In Methods in Enzymology: Single Molecule Methods, Walter, N (Ed.), Elsevier (2010)

39. J. van Mameren, P. Gross, G. Farge, P. Hooijman, M. Modesti, M. Falkenberg, E.J.G Peterman*, G.J.L. Wuite* 
Unraveling the structure of DNA during overstretching using multicolor, single-molecule fluorescence imaging
Proc. Natl. Acad. Sci. U. S. A. 106:18231-18236 (2009)
See also: Commentary by Williams et al. in PNAS in this issue highlight

38. Metzler R, Koren T, van den Broek B, Wuite GJL, Lomholt MA
And did he search for you, and could not find you?
Journal of Physics A 42(43):434005 (2009)

37. P.A. Wiggins, R.T. Dame, M.C. Noom, G.J.L. Wuite
Protein mediated bridging motifs: a key mechanism in biopolymer organization
Biophysical Journal 97(7): 1997-2003 (2009)
Selected as Featured article by Biophys Journal

36. C. Battle, B. van den Broek, M.C. Noom, J. van Mameren, G.J.L. Wuite, F.C. MacKintosh
Unraveling DNA tori under tension
Physical Review E 80(3): 031917 Part 1 (2009)

35. A. Arkhipov, W.H. Roos, G.J.L. Wuite, K. Schulten
Elucidating the mechanism behind irreversible deformation of viral capsids
Biophysical Journal 97(7): 2061-2069 (2009)

34. W.H. Roos, K. Radtke, E. Kniesmeijer, H. Geertsema, B. Sodeik, G.J.L. Wuite
Scaffold expulsion and genome packaging trigger stabilization of Herpes Simplex Virus capsids
Proc. Natl. Acad. Sci. U. S. A. 106:9673-9678 (2009)

See also: Science Daily Twitter report

33. N. Laurens, S. Bellamy, A. Harms, Y. Kovacheva, S. Halford, G.J.L. Wuite
Dissecting protein-induced DNA looping dynamics in real time
Nucleic Acids Research   (2009) doi: 10.1093/nar/gkp570

32. M. Lomholt, B. van den Broek, S.-M. Kalisch, G.J.L. Wuite, R. Metzler
Facilitated diffusion with DNA coiling
Proc. Natl. Acad. Sci. U. S. A. 106:8204-8208 (2009)

31. J. van Mameren, M. Modesti R. Kanaar, C. Wyman, E.J.G. Peterman, G.J.L. Wuite
Counting RAD51 proteins disassembling from nucleoprotein filaments under tension
Nature 457, 745 - 748 (2009), doi: 10.1038/nature07581

See also: NRC (premier Dutch newspaper), 13 Dec. 2008

30. W.H. Roos, G.J.L. Wuite
Nanoindentation studies reveal material properties of viruses
Advanced Materials  21 (10-11):1187-1192 (2009) doi: 10.1002/adma.200801709

29. D. Millo, A. Ranieri, P. Gross, H.K. Ly, M. Borsari, P. Hildebrandt, G.J.L. Wuite, C. Gooijer, G. van der Zwan
The electrochemical response of cytochrome c immobilized on smooth and roughened silver and gold surfaces chemically modified with 11-mercaptounodecanoic acid
Journal of Physical Chemistry 113 (7): 2861–2866 (2009)

28. C.I. Zoldesi, I.L. Ivanovska, C. Quilliet, G.J.L. Wuite, A. Imhof
Elastic properties of hollow colloidal particles
Physical Review E 78: 051401 (2008)

27. B. van den Broek, M.A. Lomholt, S-M.J. Kalisch, R. Metzler, G.J.L. Wuite
How DNA coiling enhances target localization by proteins
Proc. Natl. Acad. Sci. U. S. A. 105 (41):15738-15742 (2008)

See also Highlight: Nature Structural & Molecular Biology 15, 1137 (2008) 
NRC (premier Dutch newspaper), 18 Oct. 2008

26. J. van Mameren, E.J.G. Peterman, and G.J.L. Wuite
See me, feel me: methods to concurrently visualize and manipulate single DNA molecules and associated proteins
Nucleic Acids Research 36:4381-4389 (2008)

25. C. Uetrecht, C. Versluis, N. Watts, W.H. Roos, G.J.L. Wuite, P. Wingfield, A.C. Steven, A.J.R. Heck
Structure, Stability and Shape of Hepatitis B Virus Capsids
Proc. Natl. Acad. Sci. U. S. A. 105(27): 9216-9220 (2008)

24. M.C. Noom, B. van den Broek, J. van Mameren, G.J.L. Wuite
Visualizing single DNA-bound proteins using DNA as a scanning probe
Nature Methods 4, 1031 - 1036 (2007) + cover

See also Highlight; Lab on a Chip 8, 195 (2008) 

23. M.C. Noom, W.W. Navarre, T. Oshima, G.J.L. Wuite, R.T. Dame
H-NS promotes looped domain formation in the bacterial chromosome
Current Biology 17(21): R913-R914 (2007)

22. G.J.L. Wuite*, I.L. Ivanovska*, B. Jönsson, A. Evilevitch
Internal DNA pressure modifies stability of wild-type phage
Proc. Natl. Acad. Sci. U. S. A. 104:9603-9608 (2007)

21. W. Roos, I.L. Ivanovska, A. Evilevitch, G.J.L. Wuite
Viral capsids: mechanical characteristics, genome packaging and delivery mechanisms
Cellular and Molecular Life Sciences 64: 1484-1497 (2007) doi:10.1007/s00018-007-6451-1

20. M. Modesti, D. Ristic, T. v.d. Heijden, C. Dekker, J. v Mameren, E.J.G. Peterman, G.J.L. Wuite, R. Kanaar, C. Wyman Fluorescent human RAD51 reveals multiple nucleation sites and filament segments tightly associated along a single DNA molecule
Structure 15:599-609 (2007)

19. R.T. Dame, M.C. Noom, G.J.L. Wuite
Bacterial chromatin organization by H-NS protein unraveled using a dual DNA assay
Nature 444:387-390 (2006)

See also exclusive review in BioEssays 29 (3):212 - 216 (2007)
See also conference report in Nature Chemical Biology 3(4):193–197 (2007)
Extensive media coverage in the Netherlands

18. W.S. Klug, R.F. Bruinsma, J.P Michel, C.M. Knobler, I.L. Ivanovska, C.F. Schmidt, G.J.L. Wuite
Failure of Viral Shells
Physical Review Letters 97:228101 (2006)

See also News and Views; Nature Physics 3, 19 (2007); doi:10.1038/nphys500

17. J. van Mameren, M. Modesti, R. Kanaar, C. Wyman, G.J.L. Wuite, E.J.G. Peterman
Dissecting elastic heterogeneity along DNA molecules coated partly with Rad51 using concurrent fluorescence microscopy and optical tweezers
Biophysical Journal 91(8):L78-L80 (2006) doi:10.1529/biophysj.106.089466

16. M.S. Luijsterburg, M.C. Noom, G.J.L. Wuite, R.T. Dame
The architectural role of nucleoid-associated proteins in the organization of bacterial chromatin: A molecular perspective
Journal of Structural Biology 165, 262-272 (2006) doi:10.1016/j.jsb.2006.05.006  

15. J.P. Michel, I.L. Ivanovska, M.M. Gibbons, W.S. Klug, C.M. Knobler, G.J.L. Wuite, C.F. Schmidt
Nanoindentation studies of full and empty viral capsids and the effects of capsid protein mutations on elasticity and strength
Proc. Natl. Acad. Sci. U. S. A. 103:6184-6189 (2006)

14. B. van den Broek, F. Vanzi, D. Normanno, F.S. Pavone, G.J.L. Wuite
Real-time observation of DNA looping dynamics of type IIE restriction enzymes NaeI and NarI
Nucleic Acids Research 34(1) 167-174 (2006) 

13. K.C. Vermeulen, G.J.L. Wuite, G.J.M. Stienen, and C.F. Schmidt
Optical trap stiffness in the presence and absence of spherical aberrations
Appl. Opt. 45: 1812-1819 (2006)

12. K.C. Vermeulen, J. van Mameren, G.J.M. Stienen, E.J.G. Peterman, G.J.L. Wuite, C.F. Schmidt
Calibrating bead displacements in optical tweezers using acousto-optic deflectors
Review of Scientific Instruments 77:013704 (2006)

11. S.R. Leliveld, R.T. Dame, G.J.L. Wuite, Lothar Stitz, C. Korth  
Molecular basis of genetic Creutzfeldt Jakob disease linked to expansion of the prion protein octarepeat domain
J. Biol. Chem. 281 (6):3268-3275 (2006)

10. B. van den Broek, M.C. Noom, and G.J.L. Wuite
DNA-tension dependence of restriction enzyme activity reveals mechanochemical properties of the reaction pathway
Nucleic Acids Research 33(8):2676-2684 (2005)

9. R.T. Dame, J. van Mameren, M.S. Luijsterburg, M.E. Mysiak,A. Janiijevi, G. Pazdzior, P.C. van der Vliet, C. Wyman, G.J.L. Wuite
Simulation-based estimation of protein-induced DNA bending angles by scanning force microscopy
Nucleic Acids Research 33(7):e68 (2005)
 See also: Online supporting software

8. R.T. Dame, M.S. Luijsterburg, E. Krin, P.N. Bertin, R. Wagner, G.J.L. Wuite
DNA bridging: a property shared among H-NS-like proteins
J. Bacteriol. 187:1845-8 (2005)

7. I.L.Ivanovska, P.J. de Pablo, B. Ibarra, G. Sgalari, F.C. MacKintosh, J.L.Carrascosa, C.F. Schmidt, G.J.L. Wuite
Bacteriophage capsids: tough nano-shells with complex elastic properties
Proc. Natl. Acad. Sci. U. S. A. 101:7600-7605 (2004)

6. R.T.Dame and G.J.L. Wuite
On the role of H-NS in the organization of bacterial chromatin. From bulk to single molecules and back
Biophysical Journal 85:4146-4148 (2003)

5. N.Forde, D. Izhaky, G. Woodcock, G.J.L. Wuite, C. Bustamante
Using mechanical force to probe the mechanism of pausing and arrest during continuous elongation by E. coli RNA polymerase
Proc. Natl. Acad. Sci. U. S. A. 99:11682-11687(2002)

4. G.J.L. Wuite, S.B. Smith, M. Young, D. Keller, C. Bustamante
Single Molecule Studies of the Effect of Template Tension on T7 DNA Polymerase Activity 
Nature 404:103-106 (2000)

3. G.J.L. Wuite*, J. Davenport*, R. Landick, C. Bustamante
Single Molecule Study of Transcriptional Pausing and Arrest by E. coli RNA Polymerase 
Science 287:2497-2500 (2000)

2. C. Bustamante, J. Macosko, G.J.L. Wuite
Grabbing the Cat by the Tail: Manipulating Molecules One by One 
Nature Reviews Molecular Cell Biology 1:130-136 (2000) 

1. G.J.L. Wuite, J. Davenport, A. Rappaport, C. Bustamante
An Integrated Laser Trap/Flow Control Video Microscope for the Study of Single Biomolecules 
Biophysical Journal 79:1155-1167 (2000

*Equal contributions


Single Molecule Analysis, Series Methods in Molecular Biology, Vol. 783 (2011)
Peterman, E. J. G. and Wuite G. J. L. editors
Humana Press.


  1. Wuite, van den Broek, van Mameren & Noom.

  2. Wuite, Peterman, Laurens & Sitters.

  3. Wuite, Peterman, Sitters & Kamsma.
    MOLECULAR MANIPULATION SYSTEM AND METHOD (Acoustical pushing). patent pending, #PCT/NL2014/050377 (2013)

  4. Wuite, Peterman, Hell, Heller, Candelli, Sitters.
    ETHOD AND SYSTEM FOR IMAGING A MOLECULAR STRAND (I-trap). patent pending, #PCT/NL2014/050351 (2013)

  5. Wuite, Peterman, Heller, Laurens, Candelli, Biebricher.


Joost Snijder, Gijs Wuite en Wouter Roos.
Voelen en vervormen van virussen.
Ned. Tijdschr. v. Nat. 77 (3):70- (2011)

G.J.L. Wuite
Natuurkunde van het genoom
Ned. Tijdschr. v. Nat. 74 (7): 256-258 (2008)

W.H. Roos, C. Uetrecht, N. Watts, P. Wingfield, A.C. Steven, A.J.R. Heck, J.L. Carrascosa, C. F. Schmidt, G.J.L. Wuite
Force Spectroscopy reveals mechanical structure of capsids
Proceedings of the European Microscopy Congress 3:215-216 (2008)

G.J.L. Wuite
A singular view of DNA transactions
Proceedings of the micro total analysis systems (2007)

M.C. Noom, R.T. Dame, G.J.L. Wuite
Het bacterieel chromosoom ontrafeld met een viervoudig optisch pincet
Ned. Tijdschr. v. Nat. 73 (2):46-48 (2007)

G.J.L. Wuite
Single Molecule Studies of RNA and DNA Polymerases using Laser Tweezers
PhD Thesis, January 6th (2000) Advisors: Prof. J. Greve & Prof. C. Bustamante

M. Bennink and G.J.L. Wuite
The elasticity of a single DNA-molecule
Ned. Tijdschr. v. Nat. (1998)

Tuning the Music: Acoustic Force Spectroscopy (AFS) 2.0

By: Kamsma, Douwe; Creyghton, Ramon; Sitters, Gerrit; et al.

METHODS   Volume: 105   Pages: 26-33   Published: AUG 1 2016



Protein Nanocontainers from Nonviral Origin: Testing the Mechanics of Artificial and Natural Protein Cages by AFM

By: Heinze, K.; Sasaki, E.; King, N. P.; et al.

JOURNAL OF PHYSICAL CHEMISTRY B   Volume: 120   Issue: 26   Pages: 5945-5952



Atomic force microscopy observation and characterization of single virions and virus-like particles by nano-indentation

By: Marchetti, M.; Wuite, G. J. L.; Roos, W. H.

CURRENT OPINION IN VIROLOGY   Volume: 18   Pages: 82-88